| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tysinger, Alexandra N (11)
|
NCSAC |
29.59 |
29.56 |
-0.03 |
| 2 |
Diouf, Aissatou D (12)
|
NCGG |
29.97 |
30.37 |
0.40 |
| 3 |
Burns, Rachel C (11)
|
NCNMA |
40.83 |
34.44 |
-6.39 |
| 4 |
McNeal, Miller R (12)
|
NCGG |
|
34.78 |
- |
| 5 |
Sims, Ada T (11)
|
NCRCST |
38.61 |
38.16 |
-0.45 |
| 6 |
Terhardt, Kayla A (11)
|
NCRCST |
|
38.35 |
- |
| 7 |
Hernandez, Isabella A (11)
|
NCRCST |
40.23 |
43.65 |
3.42 |
| 8 |
Hollingsworth, Kendal B (11)
|
NCSAC |
44.71 |
46.32 |
1.61 |
| 9 |
Greene, Kensley H (12)
|
NCSAC |
50.88 |
46.78 |
-4.10 |
|
Royall, Addison A (11)
|
NCGG |
|
NS |
- |
|
Koo, Minah (11)
|
NCNMA |
|
NS |
- |
|
Quinn, Elektra M (12)
|
NCSAC |
44.96 |
NS |
- |